RNA Synthesis in Starved Deciliated Tetrahymena pyriformis
نویسندگان
چکیده
منابع مشابه
Ribosome Synthesis in Tetrahymena Pyriformis
The cellular site of synthesis of ribosomal RNA in Tetrahymena pyriformis was studied by analyzing the purified nuclear and cytoplasmic RNA from cells pulse labeled with uridine-(3)H. The results of studies using zonal centrifugation in sucrose density gradients show that the ribosomal RNA is synthesized in the nucleus as a large precursor molecule sedimenting at 35S. The 35S molecule undergoes...
متن کاملRegulation of ribosomal RNA synthesis in Tetrahymena pyriformis.
Ribosomal RNA is synthesized at constant rate during most of the cell cycle in heat-shock synchronized populations of Tetrahymena pyriformis. Early in each macronuclear S-period the rate of synthesis increases abruptly, concomitant with replication of the genes coding for ribosomal RNA. The increase is prevented by inhibitors of DNA replication, added prior to the S-period. Similarly, in cultur...
متن کاملSurface membrane regeneration in deciliated Tetrahymena.
The induced synthesis of identified surface membrane proteins has been demonstrated in deciliated Tetrahymena. Cells in the process of regenerating cilia were also studied using transmission electron microscopy in order to obtain information on the deployment of new membrane at the cell surface. The results obtained suggest a pattern of membrane flow that includes the 'pellicular alveoli', a su...
متن کاملRegulation of RNA synthesis in Tetrahymena pyriformis: secretion of regulatory factors.
The rate of ribosomal RNA synthesis varies greatly with the population density in both exponentially and synchronously growing populations of Tetrahymena pyriformis. Shortly after inoculation of the population - at relatively low cell densities - a gene-dose effect dominates the picture, and a doubling in the gene number is immediately followed by a doubling in the rate of RNA synthesis. Howeve...
متن کاملVincristine inhibition of DNA synthesis in Tetrahymena pyriformis.
The effect of vincristine sulfate (VCR)2 on the incorporation of isotopic precursors into protein, RNA, and DNA of the pro tozoan Tetrahymena pyriformis has been studied. DNA synthesis in logartithmically growing cells of T. pyriformi.s was almost completely inhibited by concentrations of VCR which only moderately depressed RNA and protein synthesis. Under ap propriate conditions this selective...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1979
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1979.tb13185.x